Vitamin A & Vitamin E HPLC Assay

Produktnummer ABIN2648847

Produktdetails

Target: Vitamin A & Vitamin E

Reaktivität: Chemical

Applikation: High Pressure Liquid Chromatography (HPLC)

Verwendungszweck: Vitamin A and E are fat soluble vitamins. Both are stored in the body, thus overdosing is possible. Vitamin A plays an important role in the visual process. It is mainly taken up as pre vitamin A by nutrition. In the liver it is processed to vitamin A. Retinal is involved in the visual process as prosthetic group of the rhodopsin molecule. Beside this, vitamin A (Retinol) is important for the growth of children and the growth of skin and mucous membranes. As an antioxidant, retinol protects the body against free radicals and reactive oxygen species. Overdosing leads to headache, vertigo, sickness and vomit. Disturbance of the nervous system, skin disease and loss of hair have also been reported.

Proben: Serum, Plasma

Produktmerkmale: Vitamin A and E are fat soluble vitamins. Both are stored in the body, thus overdosing is possible. Vitamin A plays an important role in the visual process. It is mainly taken up as pre vitamin A by nutrition. In the liver it is processed to vitamin A. Retinal is involved in the visual process as prosthetic group of the rhodopsin molecule. Beside this, vitamin A (Retinol) is important for the growth of children and the growth of skin and mucous membranes. As an antioxidant, retinol protects the body against free radicals and reactive oxygen species. Overdosing leads to headache, vertigo, sickness and vomit. Disturbance of the nervous system, skin disease and loss of hair have also been reported.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 250 μL

Testdauer: 0.5 h

Protokoll: For the determination of vitamin A and E, an internal standard and the precipitation reagent are added. During the precipitation step, high molecular substances are removed. After centrifugation the supernatant is injected into the HPLC system. The isocratic separation via HPLC at 30 °C uses a "reversed phase" column. One run lasts 15 minutes. The chromatograms are recorded by a UV-detector by two different wavelength (Vitamin A at 325 nm, Vitamin E 300 nm). The quantification is performed with the delivered plasma calibrator, the concentration is calculated by the "internal standard method" via integration of the peak heights resp. peak areas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Vitamin A & Vitamin E