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Thymic Stromal Lymphopoietin ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Thymic Stromal Lymphopoietin.
Produktnummer ABIN2648846

Kurzübersicht für Thymic Stromal Lymphopoietin ELISA Kit (ABIN2648846)

Target

Alle Thymic Stromal Lymphopoietin (TSLP) ELISA Kits anzeigen
Thymic Stromal Lymphopoietin (TSLP)

Reaktivität

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Human

Nachweismethode

Colorimetric

Applikation

ELISA

Proben

Serum, Plasma, Cell Culture Cells
  • Verwendungszweck

    Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Thymic Stromal Lymphopoietin (TSLP) concentrations in cell culture supernates, serum, and plasma.

    Analytische Methode

    Quantitative

    Sensitivität

    2 pg/ml

    Produktmerkmale

    Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Probenmenge

    100 μL

    Testdauer

    3.5 h

    Plattentyp

    Pre-coated

    Protokoll

    Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSLP present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TSLP is added to the wells and binds to the combination of capture antibody-TSLP in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TSLP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TSLP standard dilutions and TSLP sample concentration determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C
  • Target Alle Thymic Stromal Lymphopoietin (TSLP) ELISA Kits anzeigen

    Thymic Stromal Lymphopoietin (TSLP)

    Andere Bezeichnung

    TSLP
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