ICAM1 ELISA Kit

Produktnummer ABIN2648681

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ICAM1.

Kurzübersicht für ICAM1 ELISA Kit (ABIN2648681)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails ICAM1 ELISA Kit

Verwendungszweck: Human Intercellular Adhesion Molecule 1 (SICAM-1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Intercellular Adhesion Molecule 1 (sICAM-1) concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Sensitivität: 31 pg/ml

Produktmerkmale: Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sICAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sICAM-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for sICAM-1 is added to the wells and binds to the combination of capture antibody- sICAM-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of sICAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven sICAM-1 standard dilutions and sICAM-1 sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: sICAM-1

Substanzklasse: Viral Protein

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling