Homoarginine ELISA Kit

Produktnummer ABIN2648674

Human Homoarginine ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Homoarginine.

Kurzübersicht für Homoarginine ELISA Kit (ABIN2648674)

Target: Homoarginine

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails

Sensitivität: 0.05 μmol/L

Produktmerkmale: Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) study have identified homoarginine as useful predictor of cardiovascular events and mortality.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Testdauer: 2.5 h

Plattentyp: Pre-coated

Protokoll: The competitive Homoarginine ELISA Assay Kit uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Homoarginine

Substanzklasse: Amino Acid