GLP-1 ELISA Kit

Produktnummer ABIN2648664

Dieses Human, Maus und Ratte GLP-1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human, Maus und Ratte GLP-1 zu quantifizieren.

Kurzübersicht für GLP-1 ELISA Kit (ABIN2648664)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Bindungsspezifität: total

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Plasma

Produktdetails GLP-1 ELISA Kit

Sensitivität: 0.6 pmol/L

Produktmerkmale: The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Protokoll: Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36)/(9-36) - HRP-conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Andere Bezeichnung: GLP-1