Folic Acid ELISA Kit

Produktnummer ABIN2648657

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Chemical Folic Acid.

Kurzübersicht für Folic Acid ELISA Kit (ABIN2648657)

Target: Folic Acid (FA)

Reaktivität: Chemical

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Food

Produktdetails Folic Acid ELISA Kit

Sensitivität: 2 ng/ml

Produktmerkmale: Folates play an important role in the synthesis of nucleic acids and some amino acids and gained re-cently increased interest because they belong to the group of antioxidative vitamins. In the last years the in-fluence of folic acid supplementation to avoid abortion and dysraphism was a topic of research increasingly. Folic acid as the most stable representative of the group of folates is added to a broad range of food.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The Folic Acid ELISA Assay kit is a quantitative assay based on the principle of the enzyme linked immu-nosorbent assay. A folic acid conjugate is bound on the surface of a microtiter plate. Folic acid containing samples or standards and an antibody directed against folic acid are given into the wells of the microtiter plate. Immobi-lized and free folic acid compete for the antibody binding sites. After one hour incubation at room tem-perature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate against the antibody is given into the wells and after another hour incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the devel-opment of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of folic acid is indirectly proportional to the colour inten-sity of the test sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Folic Acid

Target: Folic Acid (FA)

Andere Bezeichnung: Folic Acid

Substanzklasse: Chemical