CXCL12 ELISA Kit

Produktnummer ABIN2648568

Dieses Human CXCL12 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CXCL12 zu quantifizieren.

Kurzübersicht für CXCL12 ELISA Kit (ABIN2648568)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma

Produktdetails CXCL12 ELISA Kit

Sensitivität: 0.31 ng/ml

Produktmerkmale: SDF-1 Alpha (CXCL12) is a CXC chemokine [1]. The small basic protein (ca. 8 kDa) has no glycosylation site in its sequence [2]. The homology properties of SDF-1 Alpha are unique among the chemokines. It has a high degree of conservation within the mammalians, with 99 % identity between the human and the murine SDF-1 Alpha sequences. But it shows a weak homology to the other CXC chemokines and the SDF-1 gene is on chromosome 10, whereas the other chemokine genes are on chromosome 4 and 17 [3].

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 50 μL

Testdauer: 2.5 h

Plattentyp: Pre-coated

Protokoll: The biotinylated antibody of the CXCL 12 / SDF-1 Alpha ELISA Assay kit binds to the streptavidin coated microtiterplate. The SDF-1 α of the standards/samples binds to this antibody and is detected by the primary antibody. The biotinylated antibody, the primary antibody and the standards/samples are incubated on the microtiterplate altogether at the same time. The primary antibody, which is bound to SDF-1 Alpha, is then detected by a secondary peroxidase-conjugated antibody. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of SDF-1 Alpha.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: CXCL 12/SDF-1 alpha

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling