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G-CSF ELISA Kit

CSF3 Reaktivität: Human Colorimetric Cell Culture Cells, Plasma, Serum
Produktnummer ABIN2648562
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Reaktivität
    • 16
    • 14
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Applikation
    ELISA
    Verwendungszweck
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Colony Stimulating Factor (G-CSF) concentrations in cell culture supernates, serum, and plasma.
    Proben
    Serum, Plasma, Cell Culture Cells
    Analytische Methode
    Quantitative
    Sensitivität
    4 pg/ml
    Produktmerkmale
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
    Top Product
    Discover our top product CSF3 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Testdauer
    3.5 h
    Plattentyp
    Pre-coated
    Protokoll
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for G-CSF is added to the wells and binds to the combination of capture antibody- G-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of G-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven G-CSF standard dilutions and G-CSF sample concentration determined.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Andere Bezeichnung
    G-CSF (CSF3 Produkte)
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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