S100A8/A9 Complex (Calprotectin) ELISA Kit

Produktnummer ABIN2648553

Dieses Human S100A8/A9 Complex (Calprotectin) ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human S100A8/A9 Complex (Calprotectin) zu quantifizieren.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) ELISA Kit (ABIN2648553)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Fecal

Produktdetails S100A8/A9 Complex (Calprotectin) ELISA Kit

Sensitivität: 5 ng/ml

Produktmerkmale: This Calprotectin ELISA Assay kit is intended for use in the quantitative determination of human calprotectin (neutrophil cytoplasmic protein S100A8/A9, MRP 8/14) levels in stool samples via Enzyme-linked immunosorbent assay. The Calprotectin ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 50 mg

Testdauer: 2 h

Protokoll: Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to calprotectin. After a short incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human calprotectin specific monoclonal antibody is added to each well. After the second incubation period, a "sandwich" of solid-phase antibody - human calprotectin - HRP conjugated monoclonal antibody" is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of fecal human calprotectin in test samples is determined directly from this standard curve of the Calprotectin ELISA Assay.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: Calprotectin

Pathways: S100 Proteine