NPPA ELISA Kit

Produktnummer ABIN2541854

Maus NPPA ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus NPPA.

Kurzübersicht für NPPA ELISA Kit (ABIN2541854)

Target: NPPA (Natriuretic Peptide A (NPPA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 12.35 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Güteklasse: Small Sample

Produktdetails NPPA ELISA Kit

Untere Nachweisgrenze: 12.35 pg/mL

Analytische Methode: Quantitative

Sensitivität: < 4.64 pg/mL

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Probenmenge: 25 µL

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.

• 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.

• 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

Testdurchführung:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Set standard, test samples and control wells.

• 2) Aliquot 25 μL of diluted standard into the standard wells.

• 3) Aliquot 25 μL of Standard Diluent buffer into control (zero) well.

• 4) Aliquot 25 μL of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.

• 5) Aliquot 25 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.

• 6) Wash 3 times.

• 7) Aliquot 25 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.

• 8) Wash 5 times.

• 9) Aliquot 25 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.

• 10) Aliquot 20 μL of Stop Solution.

• 11) Measure the OD at 450 nm.

Ergebnisberechnung:

For calculation, average the O.D.450 duplicate readings for each reference standard and each sample and substract the average control (zero) O.D.450 reading. The standard curve can be plotted as the relative O.D.450 of each reference standard solution [Y] vs. the respective concentration of each standard solution (X). The ANP concentration of the samples can be interpolated from the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Atrial Natriuretic Peptide (ANP) were were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Atrial Natriuretic Peptide (ANP) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Haltbarkeit: 6 months

Details zu NPPA

Target: NPPA (Natriuretic Peptide A (NPPA))

Andere Bezeichnung: Atrial Natriuretic Peptide