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KLK1B3 ELISA Kit

Maus KLK1B3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus KLK1B3.
Produktnummer ABIN2538281

Kurzübersicht für KLK1B3 ELISA Kit (ABIN2538281)

Target

Alle KLK1B3 Produkte
KLK1B3 (Kallikrein 1-Related Peptidase B3 (KLK1B3))

Reaktivität

Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5 pg/mL - 4000 pg/mL

Applikation

ELISA

Proben

Plasma, Serum
  • Untere Nachweisgrenze

    62.5 pg/mL

    Verwendungszweck

    Mouse Kallikrein 1-Related Peptidase B3 (KLK1B3) ELISA Kit

    Analytische Methode

    Quantitative

    Sensitivität

    37.5 pg/mL

    Bestandteile

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Benötigtes Material

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Applikationshinweise

    Optimal dilutions/concentrations should be determined by the end user.

    Kommentare

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plattentyp

    Pre-coated

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Kallikrein 1-Related Peptidase B3 (KLK1B3) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Kallikrein 1-Related Peptidase B3 (KLK1B3) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C

    Informationen zur Lagerung

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Haltbarkeit

    6 months
  • Target Alle KLK1B3 Produkte

    KLK1B3 (Kallikrein 1-Related Peptidase B3 (KLK1B3))

    Andere Bezeichnung

    Kallikrein 1-Related Peptidase B3

    Hintergrund

    Ensembl: ENSMUST00000085450

    UniProt Entry Name: K1KB3_MOUSE

    Gen-ID

    18050

    NCBI Accession

    NP_032719

    UniProt

    P00756
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