Hepcidin ELISA Kit
Kurzübersicht für Hepcidin ELISA Kit (ABIN2111986)
Target
Alle Hepcidin (HAMP) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
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Untere Nachweisgrenze
- 3.7 ng/mL
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Analytische Methode
- Quantitative
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Sensitivität
- < 1.48 ng/mL
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Bestandteile
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A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL
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Plattentyp
- Pre-coated
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Protokoll
- The Porcine Hepcidin (Hepc) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of Hepc in porcine serum, plasma, tissue homogenates, cell lysates, culture supernatants and other biological fluids.This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for Hepc has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled Hepc and unlabeled Hepc (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of Hepc in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Hepc in the sample.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C/-20 °C
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- Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
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Andere Bezeichnung
- Hepcidin (Hepc)
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Pathways
- Hormone Activity, Transition Metal Ion Homeostasis
Target Alle Hepcidin (HAMP) ELISA Kits anzeigen
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