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Hepcidin ELISA Kit

Schwein Hepcidin ELISA Kit, Colorimetric Assay für die Quantifizierung von Schwein Hepcidin.
Produktnummer ABIN2111986
1.346,32 €
Zzgl. Versandkosten 20,00 € und MwSt
96 tests
Lieferung nach: Deutschland
Lieferung in 20 bis 27 Werktagen

Kurzübersicht für Hepcidin ELISA Kit (ABIN2111986)

Target

Alle Hepcidin (HAMP) ELISA Kits anzeigen
Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reaktivität

  • 23
  • 23
  • 19
  • 9
  • 7
  • 7
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Schwein

Nachweismethode

Colorimetric

Methodentyp

Competition ELISA

Detektionsbereich

3.7-300 ng/mL

Applikation

ELISA
  • Untere Nachweisgrenze

    3.7 ng/mL

    Analytische Methode

    Quantitative

    Sensitivität

    < 1.48 ng/mL

    Bestandteile

    A: Microtiter Plate, 96 wells, Pre-coated, ready to use
    B: Standard, 2x1vial
    C: Standard Diluent, 1x20 mL
    D: Detection Reagent A, 1x120 µL
    E: Detection Reagent B, 1x120 µL
    F: Assay Diluent A, 1x10 mL
    G: Assay Diluent B, 1x10 mL
    H: TMB Substrate, 1x0 mL
    K: Stop Solution, 1x0 mL
    L: Wash Buffer, 30X, 1x20 mL
  • Plattentyp

    Pre-coated

    Protokoll

    The Porcine Hepcidin (Hepc) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of Hepc in porcine serum, plasma, tissue homogenates, cell lysates, culture supernatants and other biological fluids.This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for Hepc has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled Hepc and unlabeled Hepc (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of Hepc in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Hepc in the sample.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C/-20 °C
  • Target Alle Hepcidin (HAMP) ELISA Kits anzeigen

    Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

    Andere Bezeichnung

    Hepcidin (Hepc)

    Pathways

    Hormone Activity, Transition Metal Ion Homeostasis
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