Periostin ELISA Kit

Produktnummer ABIN2111983

Produktdetails Periostin ELISA Kit

Target: Periostin (POSTN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31-2000 pg/mL

Untere Nachweisgrenze: 31 pg/mL

Applikation: ELISA

Marke: BioAssay™

Proben: Cell Culture Supernatant, Serum, Plasma

Analytische Methode: Quantitative

Sensitivität: 10 pg/mL

Bestandteile: Mouse periostin Standard, lyophilized, 1x5ug
Detection Antibody, 1x20 µL
HRP Labeled Streptavidin, lyophilized, 1x2ug
Wash Buffer 10X, 2x30 mL
ELISA Buffer 10X, 2x30 mL
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate coated with periostin antibody, 6x16 well strips
Plate covers, 2x plastic film
Silica gel, 2x minibags

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5-- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of periostin in the samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Periostin

Target: Periostin (POSTN)

Andere Bezeichnung: Periostin (POSTN Produkte)

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Hintergrund: Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.