beta-2 Microglobulin ELISA Kit

Produktnummer ABIN2111978

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus beta-2 Microglobulin.

Kurzübersicht für beta-2 Microglobulin ELISA Kit (ABIN2111978)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 4.7-300 ng/mL

Applikation: ELISA

Produktdetails beta-2 Microglobulin ELISA Kit

Untere Nachweisgrenze: 4.7 ng/mL

Analytische Methode: Quantitative

Sensitivität: < 2.1 ng/mL

Bestandteile: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Mouse beta-2-Microglobulin (b2M) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of b2M in mouse serum, plasma, urine, tissue homogenates, cell lysates, cell culture supertantants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to b2M. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to b2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain b2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of b2M in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: beta-2 Microglobulin (b2M)

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process