ZP2 ELISA Kit

Produktnummer ABIN2044869

Das Ratte ZP2 ELISA Kit (ABIN2044869) ist ein Colorimetric ELISA Kit zur Detektion von Ratte ZP2.

Kurzübersicht für ZP2 ELISA Kit (ABIN2044869)

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ZP2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.125 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ZP2

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Andere Bezeichnung: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)