WWP2 ELISA Kit

Produktnummer ABIN2044855

Human WWP2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human WWP2.

Kurzübersicht für WWP2 ELISA Kit (ABIN2044855)

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails WWP2 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.27 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WWP2

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Pathways: Negative Regulation of Transporter Activity