WFS1 ELISA Kit

Produktnummer ABIN2044849

Human WFS1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human WFS1.

Kurzübersicht für WFS1 ELISA Kit (ABIN2044849)

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails WFS1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.103 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Wolfram Syndrome Protein 1 (WFS1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wolfram Syndrome Protein 1 (WFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wolfram Syndrome Protein 1 (WFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WFS1

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Andere Bezeichnung: Wolfram Syndrome Protein 1 (WFS1)

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom