WFDC2 ELISA Kit

Produktnummer ABIN2044803

Dieses Human WFDC2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human WFDC2 zu quantifizieren.

Kurzübersicht für WFDC2 ELISA Kit (ABIN2044803)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails WFDC2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.114 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: WAP Four Disulfide Core Domain Protein 2 (WFDC2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WAP Four Disulfide Core Domain Protein 2 (WFDC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WAP Four Disulfide Core Domain Protein 2 (WFDC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WFDC2

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Andere Bezeichnung: WAP Four Disulfide Core Domain Protein 2 (WFDC2)