Vitamin D-Binding Protein ELISA Kit
Kurzübersicht für Vitamin D-Binding Protein ELISA Kit (ABIN2044799)
Target
Alle Vitamin D-Binding Protein (GC) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
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Untere Nachweisgrenze
- 12.5 ng/mL
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Sensitivität
- < 5.57 ng/mL
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Plattentyp
- Pre-coated
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Protokoll
- Vitamin D Binding Protein (DBP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C/-20 °C
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- Vitamin D-Binding Protein (GC)
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Andere Bezeichnung
- Vitamin D Binding Protein (DBP)
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
Target Alle Vitamin D-Binding Protein (GC) ELISA Kits anzeigen
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