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Vitamin D-Binding Protein ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte Vitamin D-Binding Protein.
Produktnummer ABIN2044799

Kurzübersicht für Vitamin D-Binding Protein ELISA Kit (ABIN2044799)

Target

Alle Vitamin D-Binding Protein (GC) ELISA Kits anzeigen
Vitamin D-Binding Protein (GC)

Reaktivität

  • 15
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Ratte

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

12.5-800 ng/mL

Applikation

ELISA
  • Untere Nachweisgrenze

    12.5 ng/mL

    Sensitivität

    < 5.57 ng/mL
  • Plattentyp

    Pre-coated

    Protokoll

    Vitamin D Binding Protein (DBP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C/-20 °C
  • Target Alle Vitamin D-Binding Protein (GC) ELISA Kits anzeigen

    Vitamin D-Binding Protein (GC)

    Andere Bezeichnung

    Vitamin D Binding Protein (DBP)

    Pathways

    Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
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