NAMPT ELISA Kit

Produktnummer ABIN2044797

Dieses Meerschweinchen NAMPT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Meerschweinchen NAMPT zu quantifizieren.

Kurzübersicht für NAMPT ELISA Kit (ABIN2044797)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reaktivität: Meerschweinchen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails NAMPT ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Marke: BioAssay™

Sensitivität: < 0.52 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Visfatin (VF) BioAssay-ELISA Kit (Guinea Pig) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: Visfatin (VF)