UPRT ELISA Kit

Produktnummer ABIN2044757

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human UPRT.

Kurzübersicht für UPRT ELISA Kit (ABIN2044757)

Target: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails UPRT ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.051 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Uracil Phosphoribosyltransferase (UPRT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uracil Phosphoribosyltransferase (UPRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uracil Phosphoribosyltransferase (UPRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uracil Phosphoribosyltransferase (UPRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uracil Phosphoribosyltransferase (UPRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu UPRT

Target: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Andere Bezeichnung: Uracil Phosphoribosyltransferase (UPRT)

Pathways: Ribonucleoside Biosynthetic Process