UGT2B7 ELISA Kit

Produktnummer ABIN2044749

Dieses Human UGT2B7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human UGT2B7 zu quantifizieren.

Kurzübersicht für UGT2B7 ELISA Kit (ABIN2044749)

Target: UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails UGT2B7 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.113 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu UGT2B7

Target: UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7))

Pathways: Steroid Hormone Biosynthesis