UBR4 ELISA Kit

Produktnummer ABIN2044743

Dieses Human UBR4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human UBR4 zu quantifizieren.

Kurzübersicht für UBR4 ELISA Kit (ABIN2044743)

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails UBR4 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.053 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu UBR4

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))