ISG15 ELISA Kit

Produktnummer ABIN2044741

Human ISG15 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ISG15.

Kurzübersicht für ISG15 ELISA Kit (ABIN2044741)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails ISG15 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 13.5 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ubiquitin Cross Reactive Protein (UCRP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Cross Reactive Protein (UCRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Cross Reactive Protein (UCRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Andere Bezeichnung: Ubiquitin Cross Reactive Protein (UCRP)