UQCR11 ELISA Kit

Produktnummer ABIN2044735

Human UQCR11 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human UQCR11.

Kurzübersicht für UQCR11 ELISA Kit (ABIN2044735)

Target: UQCR11 (Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails UQCR11 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.29 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ubiquinol Cytochrome C Reductase (UQCR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase (UQCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase (UQCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquinol Cytochrome C Reductase (UQCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu UQCR11

Target: UQCR11 (Ubiquinol-Cytochrome C Reductase, Complex III Subunit XI (UQCR11))

Andere Bezeichnung: Ubiquinol Cytochrome C Reductase (UQCR)

Pathways: Proton Transport