TYR ELISA Kit

Produktnummer ABIN2044733

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Rind (Kuh) TYR.

Kurzübersicht für TYR ELISA Kit (ABIN2044733)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 ng/mL

Applikation: ELISA

Produktdetails TYR ELISA Kit

Untere Nachweisgrenze: 7.81 ng/mL

Sensitivität: < 2.5 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Tyrosinase (TYR) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosinase (TYR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosinase (TYR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosinase (TYR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tyrosinase (TYR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TYR

Target: TYR (Tyrosinase (TYR))