Tppp ELISA Kit

Produktnummer ABIN2044717

Human Tppp ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Tppp.

Kurzübersicht für Tppp ELISA Kit (ABIN2044717)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Tppp ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.132 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Tubulin Polymerization Promoting Protein (TPPP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Polymerization Promoting Protein (TPPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tubulin Polymerization Promoting Protein (TPPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))