TNNT3A ELISA Kit

Produktnummer ABIN2044715

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human TNNT3A.

Kurzübersicht für TNNT3A ELISA Kit (ABIN2044715)

Target: TNNT3A (Fast Skeletal Troponin T3a (TNNT3A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails TNNT3A ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 6.2 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Troponin T Type 3, Fast Skeletal (TNNT3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 3, Fast Skeletal (TNNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Troponin T Type 3, Fast Skeletal (TNNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TNNT3A

Target: TNNT3A (Fast Skeletal Troponin T3a (TNNT3A))

Andere Bezeichnung: Troponin T Type 3, Fast Skeletal (TNNT3)