TMEM27 ELISA Kit

Produktnummer ABIN2044709

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Hund TMEM27.

Kurzübersicht für TMEM27 ELISA Kit (ABIN2044709)

Target: TMEM27 (Transmembrane Protein 27 (TMEM27))

Reaktivität: Hund

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails TMEM27 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Transmembrane Protein 27 (TMEM27) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TMEM27

Target: TMEM27 (Transmembrane Protein 27 (TMEM27))