TGFB2 ELISA Kit

Produktnummer ABIN2044693

Ratte TGFB2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte TGFB2.

Kurzübersicht für TGFB2 ELISA Kit (ABIN2044693)

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails TGFB2 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 6.5 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Transforming Growth Factor Beta 2 (TGFb2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 2 (TGFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transforming Growth Factor Beta 2 (TGFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TGFB2

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Andere Bezeichnung: Transforming Growth Factor beta 2 (TGFb2)

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus