TFE3 ELISA Kit

Produktnummer ABIN2044683

Produktdetails TFE3 ELISA Kit

Target: TFE3 (Transcription Factor E3 (TFE3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.118 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Transcription Factor Binding To IGHM Enhancer 3 (TFE3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TFE3

Target: TFE3 (Transcription Factor E3 (TFE3))

Andere Bezeichnung: Transcription Factor Binding To IGHM Enhancer 3 (TFE3) (TFE3 Produkte)

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