CCL17 ELISA Kit
Kurzübersicht für CCL17 ELISA Kit (ABIN2044665)
Target
Alle CCL17 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
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Untere Nachweisgrenze
- 31.25 pg/mL
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Marke
- BioAssay™
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Sensitivität
- < 10.5 pg/mL
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Plattentyp
- Pre-coated
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Protokoll
- Thymus Activation Regulated Chemokine (TARC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymus Activation Regulated Chemokine (TARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymus Activation Regulated Chemokine (TARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymus Activation Regulated Chemokine (TARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Thymus Activation Regulated Chemokine (TARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C/-20 °C
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- CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))
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Andere Bezeichnung
- Thymus Activation Regulated Chemokine (TARC)
Target Alle CCL17 ELISA Kits anzeigen
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