T-Bet ELISA Kit

Produktnummer ABIN2044645

Human T-Bet ELISA Kit, Colorimetric Assay für die Quantifizierung von Human T-Bet.

Kurzübersicht für T-Bet ELISA Kit (ABIN2044645)

Target: T-Bet

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails T-Bet ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.052 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: T-Box Protein 21 (TBX21) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu T-Bet

Target: T-Bet

Andere Bezeichnung: T-Box Protein 21 (TBX21)