SNAP25 ELISA Kit

Produktnummer ABIN2044639

Human SNAP25 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human SNAP25.

Kurzübersicht für SNAP25 ELISA Kit (ABIN2044639)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SNAP25 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.051 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Synaptosomal Associated Protein 25kDa (SNAP25) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptosomal Associated Protein 25kDa (SNAP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Synaptosomal Associated Protein 25kDa (SNAP25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Andere Bezeichnung: Synaptosomal Associated Protein 25kDa (SNAP25)

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport