Superoxide Dismutases ELISA Kit

Produktnummer ABIN2044629

Das Human Superoxide Dismutases ELISA Kit (ABIN2044629) ist ein Colorimetric ELISA Kit zur Detektion von Human Superoxide Dismutases.

Kurzübersicht für Superoxide Dismutases ELISA Kit (ABIN2044629)

Target: Superoxide Dismutases

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.063 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Superoxide Dismutases (SOD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutases (SOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutases (SOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutases (SOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Superoxide Dismutases (SOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Antigendetails

Target: Superoxide Dismutases