SDHA ELISA Kit

Produktnummer ABIN2044619

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus SDHA.

Kurzübersicht für SDHA ELISA Kit (ABIN2044619)

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails SDHA ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.117 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Succinate Dehydrogenase Complex Subunit A (SDHA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit A (SDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Succinate Dehydrogenase Complex Subunit A (SDHA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SDHA

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Andere Bezeichnung: Succinate Dehydrogenase Complex Subunit A (SDHA)