S1PR3 ELISA Kit

Produktnummer ABIN2044595

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus S1PR3.

Kurzübersicht für S1PR3 ELISA Kit (ABIN2044595)

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails S1PR3 ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 27 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sphingosine 1 Phosphate Receptor 3 (S1PR3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 3 (S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sphingosine 1 Phosphate Receptor 3 (S1PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Andere Bezeichnung: Sphingosine 1 Phosphate Receptor 3 (S1PR3)