SIRPG ELISA Kit

Produktnummer ABIN2044557

Dieses Human SIRPG ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human SIRPG zu quantifizieren.

Kurzübersicht für SIRPG ELISA Kit (ABIN2044557)

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SIRPG ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Signal Regulatory Protein Gamma (SIRPg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Gamma (SIRPg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Gamma (SIRPg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Gamma (SIRPg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Signal Regulatory Protein Gamma (SIRPg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SIRPG

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Andere Bezeichnung: Signal Regulatory Protein gamma (SIRPg)