SIRPB1 ELISA Kit

Produktnummer ABIN2044555

Human SIRPB1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human SIRPB1.

Kurzübersicht für SIRPB1 ELISA Kit (ABIN2044555)

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails SIRPB1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.109 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Signal Regulatory Protein Beta 1 (SIRPb1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Beta 1 (SIRPb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Beta 1 (SIRPb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Signal Regulatory Protein Beta 1 (SIRPb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SIRPB1

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Andere Bezeichnung: Signal Regulatory Protein beta 1 (SIRPb1)