SH3BGRL ELISA Kit

Produktnummer ABIN2044543

Human SH3BGRL ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human SH3BGRL.

Kurzübersicht für SH3BGRL ELISA Kit (ABIN2044543)

Target: SH3BGRL (SH3 Domain Binding Glutamic Acid-Rich Protein Like (SH3BGRL))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SH3BGRL ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SH3BGRL

Target: SH3BGRL (SH3 Domain Binding Glutamic Acid-Rich Protein Like (SH3BGRL))

Andere Bezeichnung: SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL)