SOX1 ELISA Kit

Produktnummer ABIN2044541

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human SOX1.

Kurzübersicht für SOX1 ELISA Kit (ABIN2044541)

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SOX1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sex Determining Region Y Box Protein 1 (SOX1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Determining Region Y Box Protein 1 (SOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Determining Region Y Box Protein 1 (SOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Determining Region Y Box Protein 1 (SOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sex Determining Region Y Box Protein 1 (SOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SOX1

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Andere Bezeichnung: Sex Determining Region Y Box Protein 1 (SOX1)