SKP2 ELISA Kit

Produktnummer ABIN2044485

Dieses Human SKP2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human SKP2 zu quantifizieren.

Kurzübersicht für SKP2 ELISA Kit (ABIN2044485)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SKP2 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: S-Phase Kinase Associated Protein 2 (SKP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 2 (SKP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of S-Phase Kinase Associated Protein 2 (SKP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Andere Bezeichnung: S-Phase Kinase Associated Protein 2 (SKP2)

Pathways: Mitotic G1-G1/S Phases