RPL13A ELISA Kit

Produktnummer ABIN2044467

Dieses Human RPL13A ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human RPL13A zu quantifizieren.

Kurzübersicht für RPL13A ELISA Kit (ABIN2044467)

Target: RPL13A (Ribosomal Protein L13a (RPL13A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RPL13A ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.058 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribosomal Protein L13A (RPL13A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein L13A (RPL13A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein L13A (RPL13A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein L13A (RPL13A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribosomal Protein L13A (RPL13A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RPL13A

Target: RPL13A (Ribosomal Protein L13a (RPL13A))