RNASEH ELISA Kit

Produktnummer ABIN2044455

Dieses Human RNASEH ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human RNASEH zu quantifizieren.

Kurzübersicht für RNASEH ELISA Kit (ABIN2044455)

Target: RNASEH (Ribonuclease H (RNASEH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RNASEH ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.052 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribonuclease H (RNASEH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease H (RNASEH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease H (RNASEH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease H (RNASEH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease H (RNASEH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RNASEH

Target: RNASEH (Ribonuclease H (RNASEH))