RNASE8 ELISA Kit

Produktnummer ABIN2044453

Human RNASE8 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human RNASE8.

Kurzübersicht für RNASE8 ELISA Kit (ABIN2044453)

Target: RNASE8 (Ribonuclease, RNase A Family, 8 (RNASE8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails RNASE8 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.109 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribonuclease A8 (RNASE8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A8 (RNASE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A8 (RNASE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A8 (RNASE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A8 (RNASE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RNASE8

Target: RNASE8 (Ribonuclease, RNase A Family, 8 (RNASE8))

Andere Bezeichnung: Ribonuclease A8 (RNASE8)