RNASE6 ELISA Kit

Produktnummer ABIN2044449

Dieses Human RNASE6 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human RNASE6 zu quantifizieren.

Kurzübersicht für RNASE6 ELISA Kit (ABIN2044449)

Target: RNASE6 (Ribonuclease, RNase A Family, K6 (RNASE6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RNASE6 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribonuclease A6 (RNASE6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A6 (RNASE6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A6 (RNASE6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A6 (RNASE6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A6 (RNASE6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RNASE6

Target: RNASE6 (Ribonuclease, RNase A Family, K6 (RNASE6))

Andere Bezeichnung: Ribonuclease A6 (RNASE6)