RNASE3 ELISA Kit

Produktnummer ABIN2044447

Human RNASE3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human RNASE3.

Kurzübersicht für RNASE3 ELISA Kit (ABIN2044447)

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 39.06-2500 pg/mL

Applikation: ELISA

Produktdetails RNASE3 ELISA Kit

Untere Nachweisgrenze: 39.06 pg/mL

Sensitivität: < 17 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribonuclease A3 (RNASE3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A3 (RNASE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A3 (RNASE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A3 (RNASE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A3 (RNASE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RNASE3

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Andere Bezeichnung: Ribonuclease A3 (RNASE3)