RETNLB ELISA Kit

Produktnummer ABIN2044425

Dieses Maus RETNLB ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus RETNLB zu quantifizieren.

Kurzübersicht für RETNLB ELISA Kit (ABIN2044425)

Target: RETNLB (Resistin Like beta (RETNLB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 pg/mL

Applikation: ELISA

Produktdetails RETNLB ELISA Kit

Untere Nachweisgrenze: 3.12 pg/mL

Sensitivität: < 1.44 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Resistin Like Beta (RETNLb) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Pathways: Hormone Activity