REG3B ELISA Kit

Produktnummer ABIN2044409

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus REG3B.

Kurzübersicht für REG3B ELISA Kit (ABIN2044409)

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 9.37-600 pg/mL

Applikation: ELISA

Produktdetails REG3B ELISA Kit

Untere Nachweisgrenze: 9.37 pg/mL

Sensitivität: < 4.1 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Regenerating Islet Derived Protein 3 Beta (REG3b) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Beta (REG3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Regenerating Islet Derived Protein 3 Beta (REG3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu REG3B

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Andere Bezeichnung: Regenerating Islet Derived Protein 3 beta (REG3b)