RORC ELISA Kit

Produktnummer ABIN2044383

Dieses Ratte RORC ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte RORC zu quantifizieren.

Kurzübersicht für RORC ELISA Kit (ABIN2044383)

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RORC ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.054 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: RAR Related Orphan Receptor Gamma (RORg) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Gamma (RORg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Gamma (RORg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Gamma (RORg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RAR Related Orphan Receptor Gamma (RORg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Andere Bezeichnung: RAR Related Orphan Receptor gamma (RORg)

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway